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The Human Breast Cell Atlas


Focus Tissue Handling and Processing

Project Goal

To establish technical conditions for isolating and classifying cell types in normal breast tissue to recommend best practices for establishing the Human Breast Cell Atlas.


Results & Resources

The Human Breast Cell Atlas team tested technical parameters and developed optimized protocols for dissociating breast tissues, as detailed in their breast tissue dissociation and breast adipose scRNA-seq protocols. They developed an open-source software tool called SCOPIT to perform fast multinomial calculations and power estimations based on the frequencies of the rarest cell types and technical requirements to help researchers understand how many cells they need to sequence. Their early findings and data from 25,790 single breast epithelial cells from seven human individuals are reported in an open publication.


Investigators

Lead Investigator

Nicholas Navin
Nicholas Navin

Co-Principal Investigators

Kai Kessenbrock
Kai Kessenbrock
Devon Lawson
Devon Lawson