The Chan Zuckerberg Initiative (CZI) seeks to support up to 10 Imaging Scientists who will work at the interface of biology, microscopy hardware, and imaging software at imaging centers across the United States. “Imaging Scientists” might be engineers, physicists, mathematicians, computer scientists, or biologists who have focused on technology development in either microscopy or data analysis fields. The primary goal of the program is to increase interactions between biologists and technology experts. The Imaging Scientists will have expertise in microscopy hardware and/or imaging software. A successful “Imaging Program” will employ an Imaging Scientist who: a) works collaboratively with experimental biologists on projects at the imaging center; b) participates in courses that disseminate advanced microscopy methods and analysis; c) trains students and postdocs in imaging technology; d) participates in a network of CZI Imaging Scientists to identify needs and drive advances in the imaging field; e) attends twice-yearly CZI scientific workshops and meetings in imaging and adjacent biomedical areas. Each grant will fund salary and fringe benefits for an Imaging Scientist at the center, a modest travel and teaching budget, plus 15% indirect costs. The award period is three years plus an additional two years if the Imaging Program passes a review at year three.