Longitudinal Full-length, Single Molecule RNA Analysis in Neurodegenerative

Emerging evidence has revealed that alternative splicing is an important mechanism that contributes to transcriptomic diversity in the brain. Yet there is relatively little data on the role of RNA splicing and post-transcriptional modifications in neurodegeneration. This team will deploy cutting-edge transcriptional analyses, including single-molecule long-read sequencing technologies, on human patient-derived samples for Huntington’s disease for a longitudinal assessment of the changing RNA landscape in disease progression. These studies will deliver unique insights into the role of RNA splicing and diversity in Huntington’s disease, as well as open up new ways to consider the role of RNA biology across neurodegenerative diseases.