Mapping the Transcriptome and Proteome of Human Testis in 3D

This network will map the human testis in 3D using paired single cell analysis by mass-spectrometry, clampFISH, and RNA-sequencing.

Building off previous work, this group improved SCoPE-MS, a tool to investigate the proteome of single cells, with the ability to quantify over 1,000 proteins per cell. To generate this data, they developed nPOP, a droplet sample preparation method for single-cell proteomics. With this methodology, they collected proteomic data from approximately 2,000 testis cells. To investigate the spatial transcriptome, this group developed clampFISH protocol, a high-throughput technique that, when combined with SCoPE-MS, provides insight into the transcriptome and proteome.