An Integrated Map of the Immune System in Humans Across Tissues and Ages

The goal of this project is to generate an integrated map of cellular diversity in the human immune system across multiple tissues and the human lifespan. It will provide a resource to study mechanisms of tissue residency and the effects of aging.

A significant focus for this project was optimizing tissue processing and cell isolation techniques. With samples from seven donors across eight organs, they generated and published single-cell RNA-sequencing data, including T- and B-cell receptors. They created a complete cloud-based solution for data management, processing, and sharing and built a platform for probabilistic modeling for single-cell data. This platform includes DestVI, a convolution spatial transcriptomic tool that allows for a multi-resolution analysis that models continuous variation within cell types.