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Enhanced, Massively Parallel, and Multimodal Profiling for the Human Cell Atlas

Focus Immune

Project Goal

To establish best practices, benchmark, and provide open-source analysis tools for CITE-seq, a new technology for massively parallel and multimodal single-cell profiling.

Results & Resources

Any lab interested in implementing CITE-Seq now has access to open experimental protocols, a python tool for pre-processing multimodal data (CITE-seq-Count), integration with the Seurat R toolkit, and a vignette demonstrating the analysis workflow. Additionally, CITE-seq and CellHashing, a multiplexing approach using CITE-Seq, have preprints describing the work.


Lead Investigator

Rahul Satija
Rahul Satija

Co-Principal Investigators

Peter Smibert
Peter Smibert