In situ Interrogation of Epigenetic States in Single-Cells
To develop a transformative new assay for multiplexed, in situ analysis of epigenetic states in single mammalian cells and to use the assay to study hematopoiesis.
Results & Resources
Kueh and Vaughan have continued development of an imaging assay, SCEPTRE (Single-Cell Evaluation of Post TRanslational Epigenetic modifications), that combines superresolution imaging with spectral barcoding to enable simultaneous visualization of several different histone modifications on multiple genes in single-cells. In their ongoing work they will validate the ability to read out chromatin markers for single-cells and single-alleles.