In situ Interrogation of Epigenetic States in Single-Cells

To develop a transformative new assay for multiplexed, in situ analysis of epigenetic states in single mammalian cells and to use the assay to study hematopoiesis.

Kueh and Vaughan have continued development of an imaging assay, SCEPTRE (Single-Cell Evaluation of Post TRanslational Epigenetic modifications), that combines superresolution imaging with spectral barcoding to enable simultaneous visualization of several different histone modifications on multiple genes in single-cells. In their ongoing work they will validate the ability to read out chromatin markers for single-cells and single-alleles.