In situ Interrogation of Epigenetic States in Single-Cells
Focus
Immune
Project Goal
To develop a transformative new assay for multiplexed, in situ analysis of epigenetic states in single mammalian cells and to use the assay to study hematopoiesis.
Results & Resources
Kueh and Vaughan have continued development of an imaging assay, SCEPTRE (Single-Cell Evaluation of Post TRanslational Epigenetic modifications), that combines superresolution imaging with spectral barcoding to enable simultaneous visualization of several different histone modifications on multiple genes in single-cells. In their ongoing work they will validate the ability to read out chromatin markers for single-cells and single-alleles.
Investigators
Lead Investigator
![Joshua Vaughan](https://chanzuckerberg.com/wp-content/uploads/2020/03/Vaughan_Joshua.jpg)
Joshua Vaughan
Co-Principal Investigators
![Hao Yuan Kueh](https://chanzuckerberg.com/wp-content/uploads/2020/03/Kueh_Hao_Yuan.jpg)
Hao Yuan Kueh