Benchmarking Single-Cell RNA-sequencing Methods

To perform systematic benchmarking of single-cell RNA-sequencing methods to identify techniques suitable for the production of a Human Cell Atlas.

In this project, the Heyn lab compared molecule capture efficiency of 13 scRNA-seq protocols to systematically evaluate the power of these techniques to describe tissue complexity and their suitability for creating a cell atlas. Their work is detailed in a preprint with the analysis code used, as well as a R package for the comparison of scRNA-seq data across tools and experiments.