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Cell and Tissue Preservation to Construct a Human Cell Atlas

Focus Gastrointestinal

Project Goal

To create, optimize and share cryopreservation methods for cells comprising visceral organs like the pancreas.

Results & Resources

The Kim lab developed a controlled procedure to cryopreserve human pancreatic
islet cells. Robust assessment of these cryopreserved cells using Patch-seq revealed transcriptomics and functions are largely maintained in these cells, although less activity may be indicated by electrophysiological parameters. They developed a library of protocols that are openly available on


Lead Investigator

Seung Kim
Seung Kim