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Cell Lineage Tracing Using Somatic Microsatellite Mutations

Focus Trajectories

Project Goal

To develop and apply computational tools that utilize somatic mutations at microsatellites as genetic barcodes to reconstruct cell lineage patterns from single-cell sequencing data.

Results & Resources

The Park lab developed a computational tool, MSIprofiler, to account for the uneven allelic and genomic coverage profiles that arise due to the unavoidable genome amplification steps required to perform single-cell experiments. They have successfully used this approach to call single-nucleotide variants in single-cell whole-genome sequencing datasets.


Lead Investigator

Peter Park
Peter Park