Human Developmental Tissue Preparation for scRNA-seq and Spatial Transcriptomics
Focus
Tissue Handling and Processing
Project Goal
To investigate, compare and establish the procedures to create a Human Developmental Cell Atlas, focusing on developmental tissue from brain, lung and heart.
Results & Resources
This group established a complete sample procurement pipeline, from informed consent for full open access to tissue handling and sample processing for scRNA-seq, spatial transcriptomics and in situ sequencing, as well as open-source code for analysis. Single-cell RNAseq data of approximately 50,000 cells from the lung and heart, and ~1.5 million cells from the brain were produced and are available on the Human Cell Atlas Data Coordination Platform.
Investigators
Lead Investigator
![Sten Linnarsson](https://chanzuckerberg.com/wp-content/uploads/2020/03/Linnarsson_Sten.jpg)
Sten Linnarsson
Co-Principal Investigators
![Joakim Lundeberg](https://chanzuckerberg.com/wp-content/uploads/2020/03/Lundeberg_Joakim.jpeg)
Joakim Lundeberg
![Mats Nilsson](https://chanzuckerberg.com/wp-content/uploads/2020/03/Nilsson_Mats.jpg)
Mats Nilsson