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Human Fetal Tissue Preparation for scRNA-seq and Spatial Transcriptomics


Focus Tissue Handling and Processing

Project Goal

To investigate, compare and establish the procedures to create a Human Developmental Cell Atlas, focusing on developmental tissue from brain, lung and heart.


Results & Resources

This group established a complete sample procurement pipeline, from informed consent for full open access to tissue handling and sample processing for scRNA-seq, spatial transcriptomics and in situ sequencing, as well as open-source code for analysis. Single-cell RNAseq data of approximately 50,000 cells from the lung and heart, and ~1.5 million cells from the brain were produced and are available on the Human Cell Atlas Data Coordination Platform.


Investigators

Lead Investigator

Sten Linnarsson
Sten Linnarsson

Co-Principal Investigators

Joakim Lundeberg
Joakim Lundeberg
Mats Nilsson
Mats Nilsson