Human Developmental Tissue Preparation for scRNA-seq and Spatial Transcriptomics

To investigate, compare and establish the procedures to create a Human Developmental Cell Atlas, focusing on developmental tissue from brain, lung and heart.

This group established a complete sample procurement pipeline, from informed consent for full open access to tissue handling and sample processing for scRNA-seq, spatial transcriptomics and in situ sequencing, as well as open-source code for analysis. Single-cell RNAseq data of approximately 50,000 cells from the lung and heart, and ~1.5 million cells from the brain were produced and are available on the Human Cell Atlas Data Coordination Platform.