Multiplex Strategies for Fluorescence and Imaging Mass Cytometry for Image-Based Analyses

To refine and couple a method for the co-detection of several DNA, RNA and protein labels in the same cells with imaging tools that can assay dozens of labels concurrently.

The goal of this grant project was to extend prior work on hyperspectral imaging using a MUSE system that amplified and multiplexed single-molecule RNA and enabled protein, built around using a combination of advanced optics for light microscopy and engineering probes and oligonucleotides to enable multiplexed detection. Future work will deliver the technology for community use.