Spectral Phenotyping

To develop spectral phenotyping as a non-destructive, label-free method to identify and capture cell types based on their spectral properties.

The McMurray lab proposed that identification of cell subtypes would be possible by Fourier Transform Infrared Spectroscopy. Alignment of infrared (IR) and immunofluorescence (IF) datasets required innovations in three areas: detect-free tiling over larger areas of IR datasets, overlay and alignment of IR and IF data with cell-level precision, and registration software. They developed an experimental pipeline to measure and align IF and IR signals.