The Human Breast Cell Atlas

To establish technical conditions for isolating and classifying cell types in normal breast tissue to recommend best practices for establishing the Human Breast Cell Atlas.

The Human Breast Cell Atlas team tested technical parameters and developed optimized protocols for dissociating breast tissues, as detailed in their breast tissue dissociation and breast adipose scRNA-seq protocols. They developed an open-source software tool called SCOPIT to perform fast multinomial calculations and power estimations based on the frequencies of the rarest cell types and technical requirements to help researchers understand how many cells they need to sequence. Their early findings and data from 25,790 single breast epithelial cells from seven human individuals are reported in an open publication.