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Chan Zuckerberg Institute for Advanced Biological Imaging

Hardware Frontiers for CryoET Workshop

May 22-25, 2023

Cryo-electron tomography has made enormous strides over the past few years, and pioneering studies have shown the promise of understanding cellular architecture at the molecular scale. The CZ Imaging Institute Hardware Frontiers for CryoET Workshop discussed the challenges and opportunities in hardware developments for cryoET, including presentations on improvements in grid preparation, super-resolution light microscopy, sample milling, Cc correctors, laser phase plates, scanning transmission electron microscopy (STEM), and cameras. Watch recordings of the workshop below, and learn more about the CZ Imaging Institute at czimaginginstitute.org.

 
Workshop Opening

Session 1: Hardware Developments for CryoET

  • Chris Russo, MRC Laboratory of Molecular Biology
 
Workshop Day 1

Session 2: Sample Preparation

  • Juergen Plitzko, Max Planck Institute of Biochemistry
  • Katerina Naydenova, MRC Laboratory of Molecular Biology
  • Leeya Engel, Technion

Session 3: Sample Milling

  • Michael Grange, Rosalind Franklin Institute
  • Maud Dumoux, Rosalind Franklin Institute
  • Shan Xu, Yale University

Session 4: Cryo-CLEM

  • David DeRosier, Brandeis University
  • Peter Dahlberg, Stanford University
  • Jessica Matthias, Max Planck Institute for Medical Research
  • Srigokul Upadhyayula, University of California, Berkeley

Session 5: Cameras

  • David Agard, CZ Imaging Institute
  • Peter Denes, Lawrence Livermore National Laboratory
 
Workshop Day 2

Session 6: Laser Phase Plates

  • Rado Danev, University of Tokyo
  • Holger Müller, University of California, Berkeley
  • Armin Feist, Max Planck Institute for Multidisciplinary Sciences

Session 7: Scanning Transmission Electron Microscopy (STEM)

  • Carsten Sachse, Ernst-Ruska-Centre 3 for Structural Biology, Forschungszentrum Jülich
  • Angus Kirkland, University of Oxford
  • David Muller, Cornell University

Session 8: Transmission Electron Microscopy (TEM) Advances

  • Anchi Cheng, CZ Imaging Institute
  • Peiyi Wang, Southern University of Science and Technology
  • Thomas Juffmann, Universität Wien

Session 9: Lightning Talks

  • Sven Klumpe, Max Planck Institute of Biochemistry
  • Jeremy Axelrod, University of California, Berkeley
 
Workshop Day 3

Session 10: Impact on Biology

  • Wah Chiu, Stanford University
  • David Agard, CZ Imaging Institute

Session 11: Closing

  • Chris Russo, MRC Laboratory of Molecular Biology
  • Rado Danev, University of Tokyo
  • Bridget Carragher, CZ Imaging Institute
  • Clint Potter, CZ Imaging Institute
  • David Agard, CZ Imaging Institute