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Project

An Integrated Map of the Immune System in Humans Across Tissues and Ages


Focus Immune
Method Epigenetic Sequencing, Single Cell Transcriptomics, Spatial Proteomics

Project Summary

The goal of this project is to generate an integrated map of cellular diversity in the human immune system across multiple tissues and the human lifespan. It will provide a resource to study mechanisms of tissue residency and the effects of aging.


Results & Resources

A significant focus for this project was optimizing tissue processing and cell isolation techniques. With samples from seven donors across eight organs, they generated and published single-cell RNA-sequencing data, including T- and B-cell receptors. They created a complete cloud-based solution for data management, processing, and sharing and built a platform for probabilistic modeling for single-cell data. This platform includes DestVI, a convolution spatial transcriptomic tool that allows for a multi-resolution analysis that models continuous variation within cell types.


Investigators

Co-Principal Investigators
Donna Farber, PhD
Donna Farber, PhD
Joanne Jones, MBChB, MRCP, PhD
Joanne Jones, MBChB, MRCP, PhD

Peter Sims, PhD
Peter Sims, PhD
Roser Vento-Tormo, PhD
Roser Vento-Tormo, PhD

Nir Yosef, PhD
Nir Yosef, PhD
Muzlifah Haniffa, MBBCh, FRCP, PhD
Muzlifah Haniffa, MBBCh, FRCP, PhD

Collaborators
Menna Clatworthy, MBBCh, PhD, FRCP
Menna Clatworthy, MBBCh, PhD, FRCP
Sarah Teichmann, PhD
Sarah Teichmann, PhD